RE: [Histonet] brain tissue sections

From:"Weems, Joyce"

I would air dry the slides overnight before heating them at all. Then dry at 60C for 30 min and stain as usual. Somehow the air drying makes a difference. Good luck! jJoyce WeemsPathology ManagerSaint Joseph's Hospital of Atlanta404-851-7376404-851-7831 - Fax-----Original Message-----From: on behalf of Ryan Dominique SalazarSent: Fri 2/8/2008 3:58 AMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] brain tissue sections Hi,Please help me with my brain tissue sections, I'm having difficulties in staining them because they disintegrate during H&E staining, resulting into folded and incomplete sections into the slide.I processed the tissues 2 days after fixation using Leica ASP300S (all new reagents). I have no problem in cutting 5 u thickness during microtomy.I used adhesive pre-treated slides and Milli-Q water during orientation and fishing out in the floatation bath. I use flattening table as hot plate and heat the freshly cut slides at 62C, for not less than 10 minutes. During staining, I use 3 changes of xylene and abs. ethanol followed by 80% then 70% ethanol, all of them for 3 minutes. I used milli-Q water for hydration and washing during staining. For differentiation and bluing, I use 1% acid alcohol and ammonia water.Can you also provide me some techniques and difficulties for Perl's prussian blue and amyloid staining.Thank you very much for your help and your website which changes the lives of ordinary histologist like me.More power and God bless!       ---------------------------------Looking for last minute shopping deals?  Find them fast with Yahoo! Search._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.

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