[Histonet] vimentin

From:Melissa Mazan



We've used bd biosciences mouse monoclonal 550513 - 1:100 on FFPE lung 
tissue overnight at 4C with Alexafluor secondary for 30 min at 37C.  
We've also used the abcam chicken vimentin - works pretty well but huge 
background on westerns unless you use nitrocellulose.  Melissa

histonet-request@lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
>    1. Re: Used equipment (Renko, Heather D.)
>    2. ? vimentin IHC ab from ABR bioreagents (Kathy Cormier)
>    3. H & E stainers (Hineline, Paula)
>    4. Region III Meeting Program (Shirley Powell)
>    5. Re: H & E stainers (Rene J Buesa)
>    6. Biopsy bags (Martin, Erin)
>    7. RE: Biopsy bags (Horn, Hazel V)
>    8. Coverslipping with the Dako Autostainer (Sate Hamza)
>    9. RE: Biopsy bags (Mike Pence)
>   10. little things in histology (WWmn916@aol.com)
>   11. a reference for Streptavidin-biotin detecting system (K M)
>   12. RE: Xylene Resistant Floors (Kim Merriam)
>   13. Re: Coverslipping with the Dako Autostainer (Rene J Buesa)
>   14. H&E stainer (Blazek, Linda)
>   15. Re: little things in histology (Rene J Buesa)
>   16. Re: a reference for Streptavidin-biotin detecting system
>       (Rene J Buesa)
>   17. RE: Xylene Resistant Floors (Rene J Buesa)
>   18. Re: H&E stainer (Cynthia Robinson)
>   19. Double-staining with same secondary antibody? (Min-Han Tan)
>   20. In-service on Ht exam (Cheri Miller)
>   21. Ethanol preserved tissues (Laura Hunt)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 12 Feb 2008 12:04:20 -0600
> From: "Renko, Heather D." 
> Subject: [Histonet] Re: Used equipment
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	<40026EDDE64CDA47AB382C52619ACD3C0A09DD2B@pmc-rfd-mx01.intranet.osfnet.org>
> 	
> Content-Type: text/plain; charset=iso-8859-1
>
> I have a Microm 760X H&E stainer that we are replacing.  If you know of anyone that might want it or even for parts email me directly.
>
> Heather D. Renko, Histology Coordinator
> OSF Saint Anthony Medical Center
> 5666 East State Street
> Rockford, Illinois 61108
> Direct: 815-395-5410
> Heather.D.Renko@osfhealthcare.org
>
>
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>
> ------------------------------
>
> Message: 2
> Date: Tue, 12 Feb 2008 14:21:41 -0500
> From: "Kathy Cormier" 
> Subject: [Histonet] ? vimentin IHC ab from ABR bioreagents
> To: 
> Message-ID: <000a01c86dac$7f5fb3c0$92003712@mit.edu>
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Hi All,
>
> Has anyone tried the vimetin mouse mono ab from ABR bioreagents? I need to find a vimentin that will work on FFPE mouse tissue, and I do not want to spend $300 if the ab does not work well.... Any other ab that anyone knows works well? (yes, I checked the archives!)
>
> Thanks!
>
> Kathy
> DCM MIT
>
> ------------------------------
>
> Message: 3
> Date: Tue, 12 Feb 2008 14:02:29 -0600
> From: "Hineline, Paula" 
> Subject: [Histonet] H & E stainers
> To: 
> Message-ID:
> 	
> 	
> Content-Type: text/plain;	charset="us-ascii"
>
>  
> Hello,
> We are researching histology H&E stainers for our lab. We have gotten
> quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear
> stainer. Recommendations would be appreciated.
> Thanks 
> Paula Hineline, HT ASCP
> Pathology Laboratory
> phineline@grandpath.com
>  
>  
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 12 Feb 2008 15:17:13 -0500
> From: Shirley Powell 
> Subject: [Histonet] Region III Meeting Program
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <01MR7OTAYQ76002EXU@Macon2.Mercer.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Hi Guys, 
>
> The final program for the 2008 Region III Symposium/Convention hosted by GSH
> is up on the website at www.histosearch.com/gsh in a .pdf and .doc format,
> as well as vendor registration forms.  Print out you program and mail it in
> soon.  The hotel reservation deadline is March 3rd, so make them soon.  
>
> Thanks, 
> Shirley Powell, GSH Secretary/Registrar
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 12 Feb 2008 12:41:32 -0800 (PST)
> From: Rene J Buesa 
> Subject: Re: [Histonet] H & E stainers
> To: "Hineline, Paula" ,
> 	histonet@lists.utsouthwestern.edu
> Message-ID: <477774.54198.qm@web61222.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Get quotes (and ask for a demo) of the Sakura H&E stainer.
>   René J.
>
> "Hineline, Paula"  wrote:
>   
> Hello,
> We are researching histology H&E stainers for our lab. We have gotten
> quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear
> stainer. Recommendations would be appreciated.
> Thanks 
> Paula Hineline, HT ASCP
> Pathology Laboratory
> phineline@grandpath.com
>
>
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>
>        
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> ------------------------------
>
> Message: 6
> Date: Tue, 12 Feb 2008 13:23:51 -0800
> From: "Martin, Erin" 
> Subject: [Histonet] Biopsy bags
> To: "histonet" 
> Message-ID:
> 	
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone.
>  
> Does anyone have an inexpensive source for the nylon biosy bags?  The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size.  I hope that there is something cheaper out there...
>  
> Thanks in advance!
>  
> Erin Martin
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 12 Feb 2008 15:31:02 -0600
> From: "Horn, Hazel V" 
> Subject: RE: [Histonet] Biopsy bags
> To: "Martin, Erin" ,	"histonet"
> 	
> Message-ID:
> 	<9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B12@EMAIL.archildrens.org>
> Content-Type: text/plain;	charset="us-ascii"
>
> Hi Erin,
> I found the old fashioned paper tea bags at Sunburst Bottle Company of
> Sacremento for only 5 cents each.   The phone number is 916-929-3604.
> The cat# is   tb 1
>
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall    Slot 820
> Little Rock, AR   72202
>
> phone   501.364.4240
> fax        501.364.3912
>
> visit us on the web at:    www.archildrens.org
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,
> Erin
> Sent: Tuesday, February 12, 2008 3:24 PM
> To: histonet
> Subject: [Histonet] Biopsy bags
>
> Hi everyone.
>  
> Does anyone have an inexpensive source for the nylon biosy bags?  The
> price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for
> the small size.  I hope that there is something cheaper out there...
>  
> Thanks in advance!
>  
> Erin Martin
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 12 Feb 2008 15:41:11 -0600
> From: "Sate Hamza" 
> Subject: [Histonet] Coverslipping with the Dako Autostainer
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> 	<8854ff80802121341i1204884ar376df31d12843ad4@mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Colleagues,
>
> We use the DakoCytomation Autostainer and we are wondering what
> coverslipping instruments are being used to cover the slides with Dako slide
> labels ..
>
> Thank you ..
>
> Sate
>
>   

-- 
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Sports Medicine
Tufts Cummings School of Veterinary Medicine
200 Westboro Road
North Grafton, MA 01536
508-887-4589


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