[Histonet] sections falling off

From:"Olek Michalski"

Dear Histonetters,

a friend of mine just faced a massive sections falling off. She is doing  
Nissl staining in mouse brain (brains perfused with formalin, postfixed 3  
days, and cryosectioned) on poly-L-lysined slides. She just managed to  
pass trough xylene and decreasing grades of alcohol to water and the  
sections started to detach. We were trying to reattach them but it went  
out that sections are not flat any more. It seems like the tissue is  
rehydrated unevenly, but keeping it in water for hours didn't work. Is  
there any way to spread these sections not damaging them at the same time?  
She is likely to rescue this sections even if some work is needed.

Best regards
Olek Michalski
        Laboratory of Neurobiology
       of Development and Evolution

  Nencki Institute of Experimental Biology
  ul. Pasteura 3, 02-093 Warszawa,  Poland
  Tel. +48 22 5892268,  Fax +48 22 8225342

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