a friend of mine just faced a massive sections falling off. She is doing
Nissl staining in mouse brain (brains perfused with formalin, postfixed 3
days, and cryosectioned) on poly-L-lysined slides. She just managed to
pass trough xylene and decreasing grades of alcohol to water and the
sections started to detach. We were trying to reattach them but it went
out that sections are not flat any more. It seems like the tissue is
rehydrated unevenly, but keeping it in water for hours didn't work. Is
there any way to spread these sections not damaging them at the same time?
She is likely to rescue this sections even if some work is needed.
Laboratory of Neurobiology
of Development and Evolution
Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342
Histonet mailing list