Hi all,
I'm trying to do fluorescent immunostaining of macrophages on FFPE mouse
tissues - I have tried the BD monoclonal Mac-3 (raised in rat) - it
works well with the Vector ABC system, but cannot get it to work well
with fluorescence. Tried a simple primary, 594 conjugated secondary
approach - no signal. Then tried a biotinylated secondary followed with
streptavidin - get a messy, non-uniform signal. Does anyone have an
antibody and protocol for fluorescent labeling of macrophages on
formalin fixed paraffin embedded tissue? Many thanks - Melissa
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Sports Medicine
Tufts Cummings School of Veterinary Medicine
200 Westboro Road
North Grafton, MA 01536
508-887-4589
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