[Histonet] macrophage staining

From:Melissa Mazan

Hi all,
I'm trying to do fluorescent immunostaining of macrophages on FFPE mouse 
tissues - I have tried the BD monoclonal Mac-3 (raised in rat) - it 
works well with the Vector ABC system, but cannot get it to work well 
with fluorescence.  Tried a simple primary, 594 conjugated secondary 
approach - no signal.  Then tried a biotinylated secondary followed with 
streptavidin - get a messy, non-uniform signal.  Does anyone have an 
antibody and protocol for fluorescent labeling of macrophages on 
formalin fixed paraffin embedded tissue? Many thanks - Melissa

Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Sports Medicine
Tufts Cummings School of Veterinary Medicine
200 Westboro Road
North Grafton, MA 01536

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