Dear all,
My name is Barbara Verstraeten and I'm a first yeat phD student at the
university of Ghent, Belgium.
I work on very small zebrafish (40 hours post fertilisation 'till 6
days post fertilisation).
I want to do immunostaining on them so I embedded them in technovit
9100. Everything worked fine but now I have to cut them and put them
on slides.
My questions:
* What sort of knife do I use?
* I tried to cut them at 3 µm, but they roll up or fall apart. Hints?
* I put my sections on super frost slides. Here we coat them with
poly-L-lysine.
Does anyone have other suggestions on coating slides so that de
sections will stick very good?
Thank you very much!
Barbara Verstraeten, phD
Lab Ann Huysseune
Ghent, Belgium
mail:barbara.verstraeten@ugent.be
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