[Histonet] Technovit 9100 problems

From:Barbara Verstraeten

Dear all,

My name is Barbara Verstraeten and I'm a first yeat phD student at the  
university of Ghent, Belgium.
I work on very small zebrafish (40 hours post fertilisation 'till 6  
days post fertilisation).
I want to do immunostaining on them so I embedded them in technovit  
9100. Everything worked fine but now I have to cut them and put them  
on slides.

My questions:
* What sort of knife do I use?
* I tried to cut them at 3 Ám, but they roll up or fall apart. Hints?
* I put my sections on super frost slides. Here we coat them with  
Does anyone have other suggestions on coating slides so that de  
sections will stick very good?

Thank you very much!

Barbara Verstraeten, phD
Lab Ann Huysseune
Ghent, Belgium

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