[Histonet] Re: Histology Grade Solvents

From:"Carl Hobbs"

I agree with Allen.
Xylene is a good example, that I understand: "histological grade" is low in 
sulphur. Many years ago, I found that when I used xylene that was not low in 
sulphur, my H&Es after a week or so, showed great nuclear staining but...the 
eosin had "disappeared"! ( I cleared sections in this xylene and mounted in 
DPX that had been thinned in same xylene)
"Fit for the purpose"......I always liked that phrase ;-)
I love being a Histologist but, in context, my only regret is that I am not 
also a Bio/Chemist!
Horobin, Pearce, Lake, Kiernan...where are your Successors?

Rene, it's Friday I noticed ;-)
PS: Rene, many thanks for your tips re my wax/tissue separation problem. 
Equal thanks to Gayle. Sure, not solved but, that's my job to go figure it 
out, using helpful hints and tips from Histonet!

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