Hi everyone,
   
  Please help me on Iron staining. These are my questions:
   
  -do I need to stain quickly after heating in the hot plate?
  -very pale nuclear fast red (Sigma) stain.
  -Is this OK for my working solution?
  Hydrochloric Acid-Potassium Ferrocyanide Solution
  2% hydrochloric acid --------------------------------------------        20.0 ml
  1% potassium ferrocyanide -------------------------------------          20.0 ml

        Prepare just before use and discard after use.
  -my positive control is liver, but it seems the stain is pale, do I need to change my procedure?
                 3 changes of xylene (3 min each)
                 3 changes of abs. ETOH (3 min @)
                 70% ETOH
                 80% ETOH
                 HCl-K Ferrocyanide Working solution (30 min)
                 5 changes of distilled water
                 Nuclear fast red (3 min)
                 3 changes of distilled water
                 70% ETOH 3 dips
                 80% ETOH 3 dips
                 3 changes of Abs. ETOH (3 dips @)
                 3 changes of xylene (3 dips each)
                 Mount with entellan
   
  -Please provide for me any procedures/techniques
   
  Thanks fo your big help. Your advice regarding the brain tissue section works well.
   



       
---------------------------------
Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try it now.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet