We are going to inject a plasmid along with a reporter gene into muscle.
The plasmid that is transfected and its expression is detected after a few
days by identifying the protein coded by the reporter gene. One of the
reporter genes that will be used is the Lac Z gene (beta-galactosidase) and
it is identified using X-gal
(5-bromo-4-chloro-3-indoyl-beta-D-galactopyranosdie). The other is Green
Fluorescent Protein (GFP) which is identified by fluorescence microscopy.
What we need are methods to detect Lac Z and GFP in muscle tissue after the
transfections. Which kits have worked for you?
Thanks in advance,
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