[Histonet] Lac Z gene and GFP

From:Mary Ascenzi



Hello all
We are going to inject a plasmid along with a reporter gene into muscle. 
The plasmid that is transfected and its expression is detected after a few 
days by identifying the protein coded by the reporter gene.  One of the 
reporter genes that will be used is the Lac Z gene (beta-galactosidase) and 
it is identified using X-gal 
(5-bromo-4-chloro-3-indoyl-beta-D-galactopyranosdie).  The other is Green 
Fluorescent Protein (GFP) which is identified by fluorescence microscopy.

What we need are methods to detect Lac Z and GFP in muscle tissue after the 
transfections. Which kits have worked for you?

Thanks in advance,
Mary Ascenzi


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