Check the dewaxing step.
  René J.

nick bowden  wrote:
  Hello fellow histonetters.



Help!!! We have some strange, random, staining issues!!! we are getting
pale patchy staining which seems most noticeable in poc's and mucin type
sections, with some obvious uneven or patchy staining which recurs when
repeated. SSSooo we are thinking fixation? Processing? The Solutions are
all changed on processor and stainer, Haem and Eosin new, and staining
control can look fine, only to get more random bad staining. Any ideas would
be appreciated.maybe it's the water!! 



Baffled .

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