Re: [Histonet] PAS stain for nail fungus

From:Akemi Allison-Tacha


Hi  Kathy,
Since you too are from Portland, OR, that technique was paced down to  
most of the histotechs in OR  years ago.  If I can move the cobwebs   
from my brain around, Lee Luna gave us that tip when he came out to  
give a talk in the early 80's.  Are you still at Portland Adventist?
Akemi

Phoenix Lab Consulting
Specializing in Histology, SS, IHC, & Microarray
Madison, WI
Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Cell: 		(925) 788-0900
E-Mail:		akemiat3377@yahoo.com



On Feb 23, 2007, at 10:39 AM, Kathleen Boozer wrote:

> Nair is placed on the nail when grossed by the pathology assistant  
> for 1 hour and then cut.  The histotechs put nair on it after it is  
> faced for a couple of minutes and cut it on eith + or poly-l-Lysine  
> slides.  A PAS is done and it stays on great!
>
>>>>  02/22/2007 10:09 >>>
> Dr. Allen Smith's recent post reminds me of a question I've been  
> meaning to post to Histonet.
>
> Podiatrists are now performing biopsies of toenails with nail  
> fungus, in order to get a fungal stain (PAS is being requested, I  
> think) done. Apparently the insurance companies are now requiring a  
> biopsy or culture diagnosis of fungal disease before they'll pay  
> for the very expensive systemic drugs for nail fungus like  
> terbinafine (Lamisil).
>
> Is anyone on Histonet getting these requests? What protocols do you  
> use to cut and stain these difficult specimens?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
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