Re: [Histonet] New Microwave Cap Regulation

From:Stacey Burton

Thank you Hector.This brings me to a new question - How do I test for leakage is less than 5mW/cm2 at a distance of 5 cm from the surface?  Or what type of service to I call to help me with that?-Stacey----- Original Message ----From: Hector Hernandez To: Rene J Buesa ; "Luck, Greg D." ; Stacey Burton ; histonet@lists.utsouthwestern.eduSent: Thursday, February 22, 2007 4:19:54 PMSubject: RE: [Histonet] New Microwave Cap RegulationANP.29430   Phase 1This question doesn't state laboratory or home grade microwaves.  Microwaveshould be placed in an appropriate ventilated hood to contain airbornechemical contaminates and potential infectious agents. Microwaves usedoutside a fume hood should have an integral fume extractor that is certifiedby the manufacturer for use in a clinical laboratory.  This doesn't saymicrowave has to be laboratory grade, only the fume extractor. Thischecklist question doesn't apply if only non-hazardous reagents are used inthe device (microwave) (e.g. water, certain biological stains)  In my opinion you can use any microwave (laboratory or house microwave) aslong as you can document:1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface.2. Periodically monitor for temperature reproducibility.3. All containers used in the microwave are made from microwave-transparentmaterial/ microwave proof.Don't make it any harder than it is.Hope this clears a few things,Hector-----Original Message-----From:[] On Behalf Of Rene J BuesaSent: Thursday, February 22, 2007 2:19 PMTo: Luck, Greg D.; Stacey Burton; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] New Microwave Cap RegulationGreg:  The new CAP regulation stems from the fact that many labs used the "housemicrowaves ovens"(HMWO) also to fix and process tissues. That is where CAP,manufacturers and a MW task force intervened with the valid warning thatfixing and processing, and even staining when the solutions are alcoholic,are unsafe procedures to be done in a regular  HMWO,and that those procedures really require an instrument with a ventingcapability that will take the noxious vapors out of the lab.  To just heat water or buffers for HIER I did that for many years and noinspector ever said anything. Perhaps they have become more demandingrecently on this regulation, but for me there is nothing noxious to ventwhen only water, PBS or buffers are heated. In your house you also heatwater and there is nothing noxious about it (and we are talking about the"sacred" home environment).  Just my opinion though!  René J."Luck, Greg D."  wrote:  Hello all,Would venting or use of a "Laboratory Grade" microwave be required ifall that it is used for is to heat water or PBS or Tris buffers?Thanks, GregGreg Luck, B.S., HT(ASCP)=0AAnatomic Pathology SupervisorDeaconess Med Cntr800 W. 5th AveSpokane, WA 99204Offc 509.473.7077Fax Message-----From:[] On Behalf Of StaceyBurtonSent: Thursday, February 22, 2007 8:22 AMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] New Microwave Cap RegulationI am curious to know how labs have responding to the CAP QuestionANT.29430 which asks "Are microwave devices properly vented?".Have the labs continued to utilized their regular "kitchen model"microwaves and applied duct work to them to be vented out of thebuilding?Please comment.Thank you,Stacey Burton, H.T. ASCPPrecision Pathology ServicesSan Antonio Texas____________________________________________________________________________________Food fight? Enjoy some healthy debatein the Yahoo! Answers Food & Drink Q&A. mailing listHistonet@lists.utsouthwestern.edu mailing listHistonet@lists.utsouthwestern.edu that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu ____________________________________________________________________________________Don't get soaked.  Take a quick peak at the forecastwith the Yahoo! Search weather shortcut.
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