Re: [Histonet] Extended time in paraffin
Can you select the number of the station that you want to delay the
tissues in until processing starts? If you can, you could have the
tissues go through the formalins like usual and delay when they get to
the first alcohol station. The processor should then start up and
continue on with the processing in time for the tissues to be done
early Mon AM.
Otherwise, either option 2 or 3 would work. 2 would take less time to
Sent: Fri, 23 Feb 2007 9:14 AM
Subject: [Histonet] Extended time in paraffin
I have a problem with processing breast tissue over a weekend. Breast
run in a separate processor from our Sugical and endo specimens. We do
weekend staff. We usually run a two day delay with the processing
Sunday night and both processors ending early Monday AM.
The current guidelines suggest this delay of breast tissue sitting in
could result in over fixed breast tissue. The pathologists want to end
cycle on Saturday but we have no staff here to remove and/or embed the
The pathologists themselves would have to remove the cassettes from the
The questions are what can they do with these cassettes? Again, the
breast tissue, mainly cores or target blocks or tumor that may need IHC
FISH on diagnosis
1. Can they just leave the cassettes on the processor in warm paraffin
degrees) from Saturday until Monday?
2. Can they remove them from the paraffin and let them harden at room
temperature and then be heated, melted and properly embedded on Monday?
3. Can they put the cassettes in a container of warm paraffin "to
then let the whole container solidify and on Monday Histo melts the
removes the cassettes and embeds?
I have only found two clear commentaries.
Sheehan and Hrapchak who say extended time in paraffin will cause
hardening. There is also a reference in Carson, saying tissue should
paraffin the shortest time necessary for good infiltration as prolonged
causes shrinkage and hardening.
Can any of you offer me any further references and/or assistance or
Thank you so much for your time and assistance
Nyack Hospital, NY
Theresa Rohr, BA, HT(ASCP)
Section Head, Histology
160 North Midland Avenue
Nyack, New York 10960
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