I suspect that you are not getting all of the paraffin out of the 
sections before staining.

Geoff

nick bowden wrote:

>Hello fellow histonetters.
>
> 
>
>Help!!! We have some strange, random, staining issues!!!  we are getting
>pale patchy staining which seems most noticeable in poc's and mucin type
>sections, with some obvious uneven or patchy staining which recurs when
>repeated.  SSSooo we are thinking fixation? Processing?  The Solutions are
>all changed on processor and stainer, Haem and Eosin new, and staining
>control can look fine, only to get more random bad staining. Any ideas would
>be appreciated.maybe it's the water!!  
>
> 
>
>Baffled .
>
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>
>
>  
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
**********************************************



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