RE: [Histonet] oligo-1 for oligodendrocytes

From:"Surita Banwait"

Hi There

We use chemicon MAB1580 at a dilution of 1:200 on paraformaldehyde fixed
paraffin embedded mouse brain tissue with great success.

-Surita

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Surita Banwait
Morphology & Imaging Core
Research Associate II         
Buck Institute for Age Research
8001 Redwood Blvd.
Novato, CA 94945
415-209-2221
sbanwait@buckinstitute.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
T. Hooper
Sent: Wednesday, February 21, 2007 7:29 AM
To: Histonet
Subject: [Histonet] oligo-1 for oligodendrocytes

Dear Histonet,

Is anyone using Oligo-1 antibody from chemicon to stain mouse brain? 
If so, can you please contact me as we are having some protocol 
problems and I wanted advice (fixative, protocol etc). Otherwise, if 
anyone else is using another marker to stain oligodendrocytes in 
mouse brain please let me know (clone, source, catalog # etc).

Thanks!
-- 

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