RE: [Histonet] New Microwave Cap Regulation

From:"Hector Hernandez"

ANP.29430   Phase 1
This question doesn't state laboratory or home grade microwaves.  Microwave
should be placed in an appropriate ventilated hood to contain airborne
chemical contaminates and potential infectious agents. Microwaves used
outside a fume hood should have an integral fume extractor that is certified
by the manufacturer for use in a clinical laboratory.  This doesn't say
microwave has to be laboratory grade, only the fume extractor. This
checklist question doesn't apply if only non-hazardous reagents are used in
the device (microwave) (e.g. water, certain biological stains)  
In my opinion you can use any microwave (laboratory or house microwave) as
long as you can document:
1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface.
2. Periodically monitor for temperature reproducibility.
3. All containers used in the microwave are made from microwave-transparent
material/ microwave proof.
Don't make it any harder than it is.

Hope this clears a few things,


-----Original Message-----
[] On Behalf Of Rene J Buesa
Sent: Thursday, February 22, 2007 2:19 PM
To: Luck, Greg D.; Stacey Burton;
Subject: RE: [Histonet] New Microwave Cap Regulation

  The new CAP regulation stems from the fact that many labs used the "house
microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP,
manufacturers and a MW task force intervened with the valid warning that
fixing and processing, and even staining when the solutions are alcoholic,
are unsafe procedures to be done in a regular
  HMWO,and that those procedures really require an instrument with a venting
capability that will take the noxious vapors out of the lab.
  To just heat water or buffers for HIER I did that for many years and no
inspector ever said anything. Perhaps they have become more demanding
recently on this regulation, but for me there is nothing noxious to vent
when only water, PBS or buffers are heated. In your house you also heat
water and there is nothing noxious about it (and we are talking about the
"sacred" home environment).
  Just my opinion though!
  René J.

"Luck, Greg D."  wrote:
  Hello all,
Would venting or use of a "Laboratory Grade" microwave be required if
all that it is used for is to heat water or PBS or Tris buffers?
Thanks, Greg
Greg Luck, B.S., HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133

-----Original Message-----
[] On Behalf Of Stacey
Sent: Thursday, February 22, 2007 8:22 AM
Subject: [Histonet] New Microwave Cap Regulation

I am curious to know how labs have responding to the CAP Question
ANT.29430 which asks "Are microwave devices properly vented?".
Have the labs continued to utilized their regular "kitchen model"
microwaves and applied duct work to them to be vented out of the
Please comment.

Thank you,
Stacey Burton, H.T. ASCP
Precision Pathology Services
San Antonio Texas

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