RE: [Histonet] Melanin Bleaching Epitope retrieval and tissue lifting

From:"Edmondson David \(RBV\) NHS Christie Tr"

Hi Craig
 We went for Vector's VIP alternative chromogens when, years ago we
found the bleaching problem but when, out of the blue, I tried bleaching
the other week the sections stayed on the DAKO ChemMate  capillary gap
slides and I wondered that the problem had somehow gone. At the time of
problems we had been using gelatine chrome alum or Poly-lysine coated
So try a few coatings and see
Manchester UK

-----Original Message-----
[] On Behalf Of CRAIG
Sent: 22 February 2007 13:33
To: histonet
Subject: [Histonet] Melanin Bleaching Epitope retrieval and tissue

Dear Histonetters,

Does anybody have any tips of how to keep tissue sections on the slides
during heat mediated epitope retrieval after bleaching section with
oxalic acid and portassium permanganate.

The sections are skin and are only bleached for a matter of minutes.

Any help would be welcome.

Kind reagrds

Craig Barlow
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