RE: [Histonet] Freezing human skin biopsies for cytokine ICC

From:"Patsy Ruegg"

You might check out for cytokine antibodies that work on human

-----Original Message-----
[] On Behalf Of Jamie E
Sent: Thursday, February 15, 2007 3:40 PM
Subject: [Histonet] Freezing human skin biopsies for cytokine ICC

Hello histonetters,
                                       I hope someone can help me with a 
question about how to freeze a human skin biopsy. I am in research but am 
in collaboration with a clinical lab that will be collecting human  skin 
biopsies for me to do cytokine ICC  of TNF,IFN-g, among others and 
possibly cell makers. 
       My question is how do I instruct them in freezing the skin so that 
I get the best sample for this staining. I am going to embed the sample 
once it is in my lab for cryosectioning.  Also if people know of 
manufactures of antibodies that sell  TNF,IFN-g that work in human tissue 
that would also be helpful, I have found a few but many different 
protocols and fixations.

Any thoughts how be helpful.


Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
FAX: 508-793-4895
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