RE: [Histonet] Effect of formamide solutions on proteins

From:"Patsy Ruegg"

I am not much of a chemist-type but if the proteins are not protected by
cross-linking with formaldehyde then using any solvent especially one a
potent as formamide can really reek havoc, especially on surface proteins,
sounds like a bad idea to me, without 24hr in formalin.

-----Original Message-----
[] On Behalf Of Johnson,
Sent: Wednesday, February 28, 2007 9:54 AM
Subject: [Histonet] Effect of formamide solutions on proteins

To all you chemist-types out there,

What effect might 50% formamide solutions have on lightly formalin-fixed
proteins? I know what affect is has on DNA, but what about other
structural proteins in the cell?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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