Hi everyone! I am wondering whether anyone has looked at how much length of time in formalin affects the ability to do immunohistochemistry? For instance, I want to do a pathology study on heart valves but have hearts that have been in formalin 1 year, 2 years, 3 years and am afraid this will affect
my results. I would be interested in IHC staining of matrix markers including various collagen types, elastin, fibrillin, proteoglycans, glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl 4-hydroxylase.
 
Thank you!!
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