[Histonet] astrocytes in suspension
Hi All,
I am fairly new to using any kind of histology methods at all
(engineering background) so I was hoping someone could help me out. I
am interested in staining astrocytes that are in suspension with a
fluorescent tag so I can then compare amounts of fluorescence between
samples with a fluorometer. So, I know I would use GFAP (correct me if
I'm wrong) and a fluorescent secondary would fluoresce what was tagged
with GFAP. Would I have to fix these cells? If not, would I use some
sort of live cell stain, like GFP? Please help me out, I'm at a loss!
Thanks!
Carolyn Black
cblack@med.wayne.edu
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