[Histonet] Re: [IHCRG] Uroplakin vendor
There you go..
Good luck with the validation. This is not a very sensitive antibody.
It's good on the low-grade tumors, where it's not needed.
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories,
President, Ancillary Pathways
7000 SW 62nd Avenue, Suite Penthouse-C
Miami, FL 33143
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(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of
antibodies. Since no one antibody works best for all applications
(neutralization, blotting, ELISA, etc), we offer many different types
of antibodies to help solve this problem. Please inquire for other
applications or types of antibodies not listed below.
Uroplakin II clone AU1 (available as 50ug purified or 5ml Ready To
Use TCS format)
Mab to Uroplakin III (human/bovine)
Cat#RDI-PRO651108 $345.00/vial 5ml TCS formulation
cat#RDI-PRO610108 $410.00/1vial $375/vial 10+ 50ug
(lyophilized) with 0.5%BSA & 0.09% NaN3
Category Mouse monoclonal
Immunoglobulin Class IgG1
Form/Purification Hybridoma culture supernatant
Asymetric unit membrane
(AUM) preparation from bovine urinary bladder
Description/Specificity Mab AU1 reacts specifically with
uroplakin III present in the superficial cell layer of the
urothelium. Together with uroplakins UP 1A, UP 1b, and UP II,
uroplakin III contributes in constituting the asymetrical unti
membrane of the plaques of urothelial glycoprotein (47kD) and has
been shown to be a specific marker of terminal urothelial
differentiation (Wu et al., 1983, 1994).
Antibody AU1 strongly stains
the urothelial surface membrane in paraffin sections of human renal
pelvis, ureter, bladder, and uretha. About 60% of human transitional
cell carcinomas (including metastases) maintain focal (sometimes very
limited) expression of uroplakin III. Until know, no uroplakin
staining was found in any non-urothelial carcinoma (Moll et al 1995).
Uroplakin III may thus serve as a specific urothelial
differentiaition marker in cases of metastatic carcinomas with
unclear primary tumor
Antigen Recognized in Species (tested so far) Human, bovine, rat
Application Suitable for
· immunohistochemistry on
paraffin-embedded tissue (NOT ON FROZEN SECTIONS)
· immunoblotting (Western)
Working Dilution Ready-to-use for immunohistochemistry
1:10 for histochemistry
Incubation Time 1 h at RT for immunohistochemistry
Storage At 2-8°C
On Feb 21, 2007, at 11:27 AM, Orr, Rebecca wrote:
> Could any of you please send me your vendor for Uroplakin Antibody,
> I’m looking for a mouse monoclonal.
> Our application would be for human tissue, in a clinical lab
> (research use only would be fine)
> Becky Orr CLA,HT(ASCP)QIHC
> Assistant Manager, Anatomic Pathology
> Evanston Northwestern Healthcare
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