[Histonet] Re: [IHCRG] Uroplakin vendor

From:ancillarypath@mac.com

There you go..

Good luck with the validation. This is not a very sensitive antibody.  
It's good on the low-grade tumors, where it's not needed.

Hadi

==========================3D====
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories,
President, Ancillary Pathways
7000 SW 62nd Avenue, Suite Penthouse-C
Miami, FL 33143
Tel 305.740.4440
Fax 786.513.0175
www.vitromolecular.com
www.ancillarypath.com

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   ANTIBODIES

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of   
antibodies. Since no one antibody works best for all applications  
(neutralization, blotting, ELISA, etc), we offer many different types  
of antibodies to help solve this problem. Please inquire for other  
applications or types of antibodies not listed below.



Uroplakin II clone AU1 (available as 50ug purified or 5ml Ready To  
Use TCS format)

Mab to Uroplakin III (human/bovine)
Cat#RDI-PRO651108     $345.00/vial  5ml TCS formulation

cat#RDI-PRO610108    $410.00/1vial  $375/vial 10+   50ug  
(lyophilized) with 0.5%BSA & 0.09% NaN3



Clone                                 AU1

Category                            Mouse monoclonal

Immunoglobulin Class        IgG1

Form/Purification               Hybridoma culture supernatant

Antigen

                                            Asymetric unit membrane  
(AUM) preparation from bovine urinary bladder

Description/Specificity     Mab AU1 reacts specifically with  
uroplakin III present in the superficial cell layer of the  
urothelium. Together with uroplakins UP 1A, UP 1b, and UP II,  
uroplakin III contributes in constituting the asymetrical unti  
membrane of the plaques of urothelial glycoprotein (47kD) and has  
been shown to be a specific marker of terminal urothelial  
differentiation (Wu et al., 1983, 1994).

                                       Antibody AU1 strongly stains  
the urothelial surface membrane in paraffin sections of human renal  
pelvis, ureter, bladder, and uretha. About 60% of human transitional  
cell carcinomas (including metastases) maintain focal (sometimes very  
limited) expression of uroplakin III. Until know, no uroplakin  
staining was found in any non-urothelial carcinoma (Moll et al 1995).  
Uroplakin III may thus serve as a specific urothelial  
differentiaition marker in cases of metastatic carcinomas with  
unclear primary tumor



Antigen Recognized in Species (tested so far)     Human, bovine, rat  
(canine reported)



Application Suitable for

                                            immunohistochemistry on  
paraffin-embedded tissue (NOT ON FROZEN SECTIONS)

                                            immunoblotting (Western)



Working Dilution                Ready-to-use for immunohistochemistry  
(cat#RDI-PRO651108)

                                             1:10 for histochemistry   
(cat#RDI-PRO610108)



Incubation Time                   1 h at RT for immunohistochemistry

Storage                                 At 2-8C






On Feb 21, 2007, at 11:27 AM, Orr, Rebecca wrote:

> Could any of you please send me your vendor for Uroplakin Antibody,
>
> Im looking for a mouse monoclonal.
>
>
>
> Our application would be for human tissue, in a clinical lab  
> (research use only would be fine)
>
>
>
> Thanks
>
> Bec
>
>
>
> Becky Orr CLA,HT(ASCP)QIHC
>
> Assistant Manager, Anatomic Pathology
>
> Evanston Northwestern Healthcare
>
> 847-570-2771
>
>
>
>
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