[Histonet] PASH Staining in Rat Testes
We have successfully been doing the PASH stain in rat testes for some
time. All of a sudden we cannot get our controls to stain positive.
The controls are fixed in Modified Davidson's fixative. We have made
new solutions, checked the ph of the water, ordered new Schiff's, and
used new rat control blocks. We ran a positive colon control along with
the testes control. It stained very well. Any thoughts on what might
be the cause of our problem?
I would appreciate your feedback.
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