[Histonet] KOH in processing for Nail fungus

From:"Cheryl Kerry"

Nail biters anonymous--

I haven't been following this conversation so if this solution has already
been posted, I apologize for the repeat.

The best nail processing I've ever experienced first hand was in a Texas lab
last year and again in another lab in the Northeast--so it is very
reproducible.  They grossed the nail, soaked the cassette with the
representative sections in a low percentage (I think it was 10%) of
Potassium Hydroxide for one hour (no more than 90 minutes), rinsed and added
to the processors with all the rest.  

They cut like BUTTER to where I'd VOLUNTEER to cut nails.  They stayed on
the charged slides even during the PAS water rinses...amazing.  It did not
interfere with PAS staining but I can't speak to any IHC testing.  The only
negative I saw was if you forgot to pull them they'd miss the run and maybe
get squishy.

I'll try to find the actual percentage for you--but 10% is what sticks in my
head--must be all the cobwebs?

Happy Friday!


Cheryl Kerry, HT(ASCP)
Full Staff Inc.
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