Sorry just thought some additional info might be useful;
 
The pancreas tissue was frozen in OCT in a bath of isopentane on dry
ice, 10um sections were cut, air dried for 1hr, then fixed in acetone
for 10mins. I quenched endogenous peroxidase with Pierce Peroxidase
Suppressor.
Sections without primary or secondary antibodies still have a littel
background but its worse with the secondary (I have tried rabbit
anti-rat:Biotin and it was worse).
 
Ta
 


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