Yes, it makes a difference how long the tissue was in formalin, especially
as you say for years.
I would recommand to stain the tissue first with an antibody, that has to
have a positiv result and look how the tissue reacts.
In a workshop the trainer showed us biopsies for a study, that were in
formalin for years. She had to do a very crued antigenretrieval to get the
ihc working (I'm sorry I don't remember the detailled protocol).

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754


-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stephens,
Elizabeth Humes
Gesendet: Samstag, 24. Februar 2007 01:22
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] varying time in formalin

Hi everyone! I am wondering whether anyone has looked at how much length of
time in formalin affects the ability to do immunohistochemistry? For
instance, I want to do a pathology study on heart valves but have hearts
that have been in formalin 1 year, 2 years, 3 years and am afraid this will
affect
my results. I would be interested in IHC staining of matrix markers
including various collagen types, elastin, fibrillin, proteoglycans,
glycoaminoglycans, smooth muscle alpha actin, lysyl oxidase, prolyl
4-hydroxylase.
 
Thank you!!
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