Re: [Histonet] surgical brain specimens

From:LuAnn Anderson

Hi Rene and Paula,

I have been using 5 micron sections of brain for 
Bielschowski Silver stain for the last 15 years. 
The tangles show up just fine. I do let my 
special's slides air dry overnight though. I also 
use a small amount of gelatin in my water bath 
and use regular, non charged slides. If I cut 
H&E's and need to stain the same day, I let them 
dry well at RT or in a 40 degree drying oven 
before putting in 60 degree oven and staining. I 
rarely (can't remember the last time) lose tissue.

LuAnn Anderson HT(ASCP)
Neuropathology Lab
University of MInnesota


At 10:57 AM 2/14/2007, Rene J Buesa wrote:
>Paula:
>   You ask about better sections or better retention?
>   We used to section brain at more than 5 Ám, 
> specially if there was the idea of silver 
> staining for Alzheimer's; 5 Ám are too thin to visualize the tangles.
>   For retention we always stained the slides 
> the next day they were cut. The slides were 
> placed vertical on the smaller side for about 1 
> hour before being put in the trays, that were 
> left outside the oven during the night. The 
> next day they were placed in the oven at 60║C 
> for 30 minutes before being stained (either routine ot HC).
>   RenÚ J.
>
>"Conlon, Paula F."  wrote:
>   Hi Histonetters, We are receiving more surgical brain sections than we
>have in the past. We process the specimens on the Pathos Automatic
>Microwave Histoprocessor and we cut the sections at 5 microns. The
>sections sometimes do not stay on the slides even though we use Plus
>slides. The Neuropathologist is not happy and she has asked me to find
>out if there is some way to get better sections. Thank you in advance
>for your help.
>
>Paula Conlon
>
>Histology Supervisor
>
>Lahey Clinic
>
>41 Mall Road
>
>Burlington, MA
>
>01805
>
>781-744-1345
>
>
>
>
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