Re: [Histonet] surgical brain specimens
Hi Rene and Paula,
I have been using 5 micron sections of brain for
Bielschowski Silver stain for the last 15 years.
The tangles show up just fine. I do let my
special's slides air dry overnight though. I also
use a small amount of gelatin in my water bath
and use regular, non charged slides. If I cut
H&E's and need to stain the same day, I let them
dry well at RT or in a 40 degree drying oven
before putting in 60 degree oven and staining. I
rarely (can't remember the last time) lose tissue.
LuAnn Anderson HT(ASCP)
University of MInnesota
At 10:57 AM 2/14/2007, Rene J Buesa wrote:
> You ask about better sections or better retention?
> We used to section brain at more than 5 Ám,
> specially if there was the idea of silver
> staining for Alzheimer's; 5 Ám are too thin to visualize the tangles.
> For retention we always stained the slides
> the next day they were cut. The slides were
> placed vertical on the smaller side for about 1
> hour before being put in the trays, that were
> left outside the oven during the night. The
> next day they were placed in the oven at 60║C
> for 30 minutes before being stained (either routine ot HC).
> RenÚ J.
>"Conlon, Paula F." wrote:
> Hi Histonetters, We are receiving more surgical brain sections than we
>have in the past. We process the specimens on the Pathos Automatic
>Microwave Histoprocessor and we cut the sections at 5 microns. The
>sections sometimes do not stay on the slides even though we use Plus
>slides. The Neuropathologist is not happy and she has asked me to find
>out if there is some way to get better sections. Thank you in advance
>for your help.
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