Re: [Histonet] Animal Tissue
What animal tissue are you using? I recall learning from HistoNet a
while back that rodent tissues should be processed for shorter periods
(30 min. per solution and paraffin change compared to 60 min.) because
of potential hardening issues. This is also noted in several mouse/rat
or small animal processing schedules provided in the Animal Processing
Manual published by the National Society for Histotechnology's
Veterinary, Industry and Research Committee, edited by Gayle Callis and
Diane Sterchi (along with many other helpful tips for non-human tissue
handling!). I think it (or an updated version) is still available from
NSH (Gayle, Diane?).
Esther Peters, Ph.D.
George Mason University
> I need to get some input on cutting animal tissue. I have always worked in a
> clinical setting and have recently moved into a research lab. I find that the
> tissue is very brittle and needs to be iced for an extremely amount of time.
> Can any of you animal cutting histo techs please give me some advice on what
> type of paraffin you are using and what processing schedule you are using. I
> have recently changed to Richard Allen type 9 paraffin and it seems to have
> helped some. I would appreciate any advice you can give me.
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