RE: [Histonet] formalin fixed mouse ears-help

From:"patsy ruegg"

Cartilage does separate from the rest of the tissue very easily, you may
need to process in GMA, even in GMA it can be a problem.  I had to make
elmers glue coated slides (dip in a 5% solution in dih20, then airdry slides
overnight before using them to pick up sentions) dry the sections flat at
37dc overnight.

-----Original Message-----
[] On Behalf Of Mary
Sent: Thursday, February 08, 2007 3:57 PM
Subject: [Histonet] formalin fixed mouse ears-help

Hi there,


I'm having problems with 10% neutral buffered formalin fixed mouse ears
embedded in paraffin. They are splitting from the cartilage as soon, and
I mean, as soon as they hit the water bath.


I'm open to any suggestions, no matter how silly or simple. This is a
chronic problem and these mouse ears will continue to be submitted to me
for a long time.


Please help!


-Mary B


I am using the free version of SPAMfighter for private users.
It has removed 78 spam emails to date.
Paying users do not have this message in their emails.
Try SPAMfighter   for free now!

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>