There are a number of solvents that will dissolve PMMA without harming the tissue, including toluene, xylene, chloroform, dichloromethane and ethyl acetate. Some work faster than others, and of course they work faster warm than cold, but keep in mind that they are all flammable. My preference is 2-methoxyethyl acetate, which works relatively rapidly. I use it both for deplasticizing sections before staining, and occasionally for dissolving whole blocks.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of caron fournier
> Sent: 	Friday, February 9, 2007 11:17 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] Re: PMMA removal
> 
> I was wondering if anyone has a method for removing PMMA from bone without harming the bone. Thank you for your help.
>  
> Caron Fournier, BSc, R.T.
> 
> 
> Department of Orthopaedics,
> 
> 
> Division of Orthopaedic Engineering Research,
> 
> 
> U.B.C.
> 
> 
>  
> 
> 
> 
> -----
> 
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