RE: [Histonet] Re:Osteoblast staining

From:"Rittman, Barry R"

I believe that with most types of fixation and processing that alkaline
phosphatase is destroyed.
However, if memory serves me correctly, a method was used in which
tissue was fixed in acetone and straight into wax.
Short time in wax.
Believe that it was stated that 70% of the alkaline phosphatase was
I did carry out this method on kidney and it was successful.
However, tissue is difficult to cut when fixed and processed in this

-----Original Message-----
[] On Behalf Of
Sent: Thursday, February 08, 2007 2:31 PM
Subject: [Histonet] Re:Osteoblast staining

I don't have a good answer, but instead a question.  Does Alkaline  
phosphatase survive de-cal and paraffin processing?  It seems that
all/most  enzymatic 
activity would be killed by this processing.....

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital

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