RE: [Histonet] Aquaporin 2

From:"Walters, Katherine S"

Hi Adam,

I have done quite a bit of Aquaporin labeling (1,2,3,&4).  I tried Alpha
Diagnostic (AQP23-A), Chemicon (AB3274-50ul) and Santa Cruz (sc-9880).
I had no luck with Alpha Diagnostics or Santa Cruz, but very good
labeling with the Chemicon antibody.  I use it at 1:500 with heat
retrieval on paraffin sections.
I hope this will save what's left of your hair, I lost a little myself
with this one!!


-----Original Message-----
[] On Behalf Of Adam
Sent: Thursday, February 15, 2007 9:00 AM
Subject: [Histonet] Aquaporin 2

Hi there Histonetters,

I am working human tissue and trying to use the Abcam aquaporin 2 ab
(15081). To date I have had absolutely no staining in
glycolmethacrylate-embedded tissue. Does anyone have any experience with
this specific ab, or are you using the millipore/chemicon one? and if
have success, what preperation have you used for the tissue? I'm
starting to
lose my hair!!!


Adam Kirk

Dr Adam Kirk MRCP
Renal/GIM Registrar
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