[Histonet] primary incubation times


I remember reading in one of the early immunohistochemistry texts that 
the reaction between a primary antibody and its epitope was believed to 
require 96 hrs. at 4 deg. C to reach equilibration.  Whether or not 
that's true for your antibodies, it is the kind of precedent that 
underlies the procedures typically used today.
The other issue is penetration--if you need tissue to be evenly labeled 
throughout the depth of the specimen then section thickness and 
embedding material may require long incubations (at least).  If surface 
labeling of an abundant epitope is enough to tell you what you want to 
know, a good primary may require less than half an hour!
Happy histo,
Mike King
UF Neuroscience
Date: Wed, 14 Feb 2007 15:24:53 -0800
From: "Patrick Laurie" 
Subject: [Histonet] Reason for lengthy primary antibody incubation?
To: "histonet@lists.utsouthwestern.edu"
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