[Histonet] Re: frozens on fat
In 1970 I worked with a research histochemist, Bob Wyllie of blest memory, an
Australian who I wish had published some of the things he knew.
He took one of the old 1960's Damon-IEC black-box cryostats, cleaned all the
lubricant out of it, and lubricated it (daily, admittedly an inconvenience)
with a silicone lubricant, packed the cryostat with dry ice, and cut thin
sections of straight fat.
I may have the specs for the lubricant around here someplace - I tried to
steal all his secrets that year - he was very helpful about that - Only English
speaker I ever met who could cuss in Vietnamese.
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