Sonya,
Have you tried an amplification system for your secondary?  If you  gave a 
bit more detail on your staining protocol, it would be helpful for  
trouble-shooting......
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet