[Histonet] Freezing human skin biopsies for cytokine ICC
I hope someone can help me with a
question about how to freeze a human skin biopsy. I am in research but am
in collaboration with a clinical lab that will be collecting human skin
biopsies for me to do cytokine ICC of TNF,IFN-g, among others and
possibly cell makers.
My question is how do I instruct them in freezing the skin so that
I get the best sample for this staining. I am going to embed the sample
once it is in my lab for cryosectioning. Also if people know of
manufactures of antibodies that sell TNF,IFN-g that work in human tissue
that would also be helpful, I have found a few but many different
protocols and fixations.
Any thoughts how be helpful.
Sr. Research Associate
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
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