Re: [Histonet] staining collagen
add 0,5g Anilinblue or methylenblue to 500ml saturated aquous solution of
everything else is done like van Gieson
Result: collagen, basement membranes and reticulin blue; cytoplasm yellow
John Kiernanīs book says reticulin and basement membranes are not stained if
you use indigocarmine (0,5 g in 200ml) collagen should be blue-green in this
----- Original Message -----
From: "Marc Tjwa"
To: "Barbara Bublava"
Sent: Sunday, February 26, 2006 4:32 PM
Subject: Re: [Histonet] staining collagen
I am also interested in the protocol that you
have suggested. Would you please be so kind to
send me the protocol by mail?
Tnx in advance
>You could use anilineblue instead of acid fuchsin. makes better contrast
>and is described in John Kiernans book
>tell me if you canīt find a protocol
>----- Original Message -----
>From: "Lobke De Bels"
>Sent: Friday, February 24, 2006 8:58 AM
>Subject: [Histonet] staining collagen
>Von Gieson gives a good contrast for the human eye, but the computer can't
>a difference between yellow/orange and red.
>If I only use acid fushine (or aniline) everything stains pink(blue).
>I have to find a way to stain the connective tissue so the computer can
>make a difference between connective tissue and the other tissue.
>The ideal should be a method that only stains collagen.
>Many thanks in advance for any help
>Lobke De Bels
>Department of Morphology
>Faculty of Veterinary Medicine
Marc Tjwa, MD
Center for Transgene Technology and Gene Therapy (CTG)
Flanders Interuniversity Institute for Biotechnology (VIB)
University of Leuven (KUL)
Campus Gasthuisberg, Herestraat 49
B-3000, Leuven, Belgium
Tel: +32-16-345775; Fax: +32-16-345990
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