Lobke:
  Why don't you try using some colour complementary filters between the light source and the slide?
  René J.

Lobke De Bels  wrote:
  
Von Gieson gives a good contrast for the human eye, but the computer can't make
a difference between yellow/orange and red.
If I only use acid fushine (or aniline) everything stains pink(blue).

I have to find a way to stain the connective tissue so the computer can clearly
make a difference between connective tissue and the other tissue.

The ideal should be a method that only stains collagen.


Many thanks in advance for any help



Lobke De Bels
Department of Morphology
Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133
9820 Merelbeke
Belgium
lobke.debels@ugent.be


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