Re: [Histonet] PGP 9.5

From:"Andi Kappeler"

Hi Peggy

we use mo-a-PGP 9.5, clone 13C4, Biomeda Cat Nr. V3231. Working conc. is 1.5 
ug Ig/ml (corresponding to 1:200), pretreatment HIER (microwave) citrate, on 
FFPE tissue, cut at 2-3 um. Working conc. will depend on visualization 
system used. In our lab we visualize with StrABC method (either with 
peroxidase - DAB or alkaline phosphatase - new fuchsin, depending on 
specificproject). Hope this helps.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Lee & Peggy Wenk" 
Sent: Wednesday, February 22, 2006 1:32 AM
Subject: [Histonet] PGP 9.5

> Anyone have experience with PGP 9.5 (Protein Gene Product)? It 
> demonstrates
> nerve fibers. One of our pathologists wants to use it on skin biopsies for
> diagnosis of neuropathies.
> We would prefer peroxidase for paraffin sections, rather than FITC or FS.
> Just wondering if people are having better luck with monoclonals or
> polyclonals, and if there is a difference in animal species for the 
> primary
> (we'll be doing this on human skin).
> Also - all the papers we find mention thick sections and using a confocal
> microscope. Anyone doing PGP on 5 um sections and/or with a regular light
> microscope?
> One of my students is doing this as her research project. She's done a lot
> of reading on it, and most papers don't discuss the actual procedure or 
> the
> type of antibody, etc. They are on the research aspect, or the diagnostic
> aspect, not on what histotechs need to do the procedures. Those articles
> that do mention techniques have very different methods (of course), and 
> are
> missing important information needed by histotechs.
> We would appreciate a couple of little hints of what works for "real life
> histotechs", to get us started in the right direction for use in a routine
> clinical immunohistochemistry lab.
> Feel free to contact me directly. Vendors included. I'll summarize and 
> relay
> the info back to Histonet.
> Thanks in advance.
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
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