Re: [Histonet] H&E staining
10 - 15 seconds in distillate water
Quick steps for 50, 75, 95% ETOH (they remove Eosin)
About 20 - 30 seconds for 100% ETOH ( for each step of two. You could stop
longer in this alcohol than the other ones above)
Pay attention!!! That procedure is coming from me: an amateur naturalist.
I think it would be better for you to lissen the opinions of the "guru"
Histologysts of this ML too!!!
----- Original Message -----
From: "eileen dusek"
Sent: Monday, February 27, 2006 5:30 PM
Subject: [Histonet] H&E staining
> Hi Everyone,
> This is a basic question.
> How long are tech leaving slides in 95% and 100% ETOH after eosin. I feel
> we are in too long but would like to get other ideas.
> Our slides have been very washed out, little to no contrast.
> I appreciate your help
> Eileen C. Dusek
> Edward Hospital
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