Re: [Histonet] Clearing Colon Fat to Reveal Lymph Nodes

From:Rene J Buesa

Sandy:
  I used the following solution to harden the lymph nodes and make them more evident to tact (more "revealed"):
  a- 100% ethanol -------- 2.0 litres
  b- distilled water -------- 0.68 litres
  c- 40% formalin --------- 0.32 litres
  d- acetic acid ------------ 0.2 litres 
  (Total volume = 3.2 litres)
  It worked quite well!
  Hope this will help you!
René J.
sandosis@uia.net wrote:
  Hello,

Could you folks please let me know what methods and reagents you have 
worked with that do a decent job of overnight clearing the fat from 
colonectomy specimens to further reveal lymph nodes? 

Many Thanks,
Sandy

---------------------------------------------
This message was sent using the UIA Web Mail Server.
ULTIMATE Internet Access, Inc http://www.uia.net/



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




		
---------------------------------
Yahoo! Mail
Bring photos to life! New PhotoMail  makes sharing a breeze. 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>