Re: [Histonet] Clearing Colon Fat to Reveal Lymph Nodes
Sandy:
I used the following solution to harden the lymph nodes and make them more evident to tact (more "revealed"):
a- 100% ethanol -------- 2.0 litres
b- distilled water -------- 0.68 litres
c- 40% formalin --------- 0.32 litres
d- acetic acid ------------ 0.2 litres
(Total volume = 3.2 litres)
It worked quite well!
Hope this will help you!
René J.
sandosis@uia.net wrote:
Hello,
Could you folks please let me know what methods and reagents you have
worked with that do a decent job of overnight clearing the fat from
colonectomy specimens to further reveal lymph nodes?
Many Thanks,
Sandy
---------------------------------------------
This message was sent using the UIA Web Mail Server.
ULTIMATE Internet Access, Inc http://www.uia.net/
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! Mail
Bring photos to life! New PhotoMail makes sharing a breeze.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>