Re: [Histonet] Clearing Colon Fat to Reveal Lymph Nodes

From:Rene J Buesa

  I used the following solution to harden the lymph nodes and make them more evident to tact (more "revealed"):
  a- 100% ethanol -------- 2.0 litres
  b- distilled water -------- 0.68 litres
  c- 40% formalin --------- 0.32 litres
  d- acetic acid ------------ 0.2 litres 
  (Total volume = 3.2 litres)
  It worked quite well!
  Hope this will help you!
René J. wrote:

Could you folks please let me know what methods and reagents you have 
worked with that do a decent job of overnight clearing the fat from 
colonectomy specimens to further reveal lymph nodes? 

Many Thanks,

This message was sent using the UIA Web Mail Server.
ULTIMATE Internet Access, Inc

Histonet mailing list

Yahoo! Mail
Bring photos to life! New PhotoMail  makes sharing a breeze. 
Histonet mailing list

<< Previous Message | Next Message >>