RE: [Histonet] Revealing lymph nodes

From:"Marshall Terry Dr, Consultant Histopathologist"

This is an opportune discussion, because I have tried Davidson's in the past and could see no difference whatsoever between before and after.
In the last few days we have made up a new batch, modified slightly to take into consideration JK's view that there should be a greater proportion of alcohol.
Same result, no difference whatsoever. No clearing, no lymph nodes going white - nothing.

Is this the Emperor's new cloths?

PS I only tried it in the first place because of the regard I have for anything uttered by Bob Richmond.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: []
Sent: 28 February 2006 18:58
Subject: [Histonet] Revealing lymph nodes

I think that a clearing fixative (such as the examples already given; there are also commercial products) is essential for finding the small lymph nodes that are often positive for colon cancer in colon resection specimens. Remember that just one tiny positive lymph node upstages the tumor, and makes chemotherapy mandatory - so there's a real patient care decision involved here.
In my travels as a locum tenens pathologist I noted that about half of pathologists were using clearing fixatives, and about half were not. As far as I know there have been no official pronouncements on the subject.
Clearing fixatives are less necessary in axillary dissection material for breast cancer - the specimensare smaller and the lymph nodes - and metastases - are usually much bigger. The mesenteries from a colon cancer resection are voluminous and hard to search through.
Bob Richmond
Gastonia NC
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