The responses suggest that some people (most, probably) are using an
alcoholic eosin solution and others are using aqueous eosin.  We use
eosin/phloxine in 80% ethanol, followed by 20 dips in one 95% ethanol and 20
dips in each of three absolute alcohols, then into xylene.  "Dips" is not a
very precise measurement, but our rate is about 2 dips per second, so the
slides are in each dish about 10 seconds.  This provides a total of 30
seconds immersion in absolute alcohol with constant agitation, which is
plenty to dehydrate a slice of tissue 5 microns thick.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of
> eileen dusek
> Sent: 	Monday, February 27, 2006 8:30 AM
> To: 	histonet
> Subject: 	[Histonet] H&E staining
> 
> Hi Everyone,
>   This is a basic question.
>   How long are tech leaving slides in 95% and 100% ETOH after eosin. I
> feel we are in too long but would like to get other ideas.
>   Our slides have been very washed out, little to no contrast.
>    
>   I appreciate your help
>    
>   Eileen C. Dusek
>   Edward Hospital
> 
> 		
> ---------------------------------
> Yahoo! Mail
> Bring photos to life! New PhotoMail  makes sharing a breeze. 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet