RE: [Histonet] Bone Marrow Decaling for IHC

From:Shirley Powell

I recommend using 5% formic acid.  Although in the past I did IHC on bones
decaled in RDO with very nice results.  Be sure you have adequate fixation

Shirley Powell 

-----Original Message-----
[] On Behalf Of
Sent: Wednesday, March 01, 2006 4:35 PM
Subject: [Histonet] Bone Marrow Decaling for IHC


Does anyone have sugestions for a "gentle" yet "effective" method of
decaling bone marrow cores so that the antigenic sites remain viable?

Thank you,

PS Thanks for all the response on clearing colon fat to reveal lymph nodes,
we are going to try the PenFix first as we are already set for the haz.
waste disposal for that reagent. 

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