I recommend using 5% formic acid.  Although in the past I did IHC on bones
decaled in RDO with very nice results.  Be sure you have adequate fixation
first.

Shirley Powell 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
sandosis@uia.net
Sent: Wednesday, March 01, 2006 4:35 PM
To: histonet@lists.utsouthwestern.edu
Cc: mark.lones@stjoe.edu
Subject: [Histonet] Bone Marrow Decaling for IHC

Hello,

Does anyone have sugestions for a "gentle" yet "effective" method of
decaling bone marrow cores so that the antigenic sites remain viable?

Thank you,
Sandy

PS Thanks for all the response on clearing colon fat to reveal lymph nodes,
we are going to try the PenFix first as we are already set for the haz.
waste disposal for that reagent. 


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