RE: [Histonet] Alcian Blue deposit

From:"Luck, Greg D."

Might try laying the slides down flat, cover with a slightly moist (HOH)
sheet of filter paper (e.g. #40's Whatman) and saturate filter paper with
your Alcian Blue working solution for your standard staining time.  That's
what we do to solve a similar problem with our Gram stain.  Good luck, Greg

-----Original Message-----
Sent: Thursday, March 02, 2006 7:37 AM
Subject: [Histonet] Alcian Blue deposit


Does anyone have any ideas to reduce alcian blue deposit on sections.  We
filter the solution after making up as well as immediatly before use.  


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