[Histonet] RE: Revealing lymph nodes

From:"Kellar, Eric C"


Hartman's fixative (formerly Davidson's fixative)works about the best for me. It is commercially available from MarketLab and BBC Biochemical to name a few.  To prepare it yourself, mix the following -

	95% ETOH			1200 ml
	37% Formaldehyde		800 ml
	DH20				1200 ml
	Glacial Acetic Acid	400 ml

The tissue can remain in the fixative for up to 24 hours. Transfer to 10% NBF. The nodes begin to turn opaque-white and reveal themselves after about 30 minutes in this fixative.

Eric C. Kellar
Quest Diagnostics Inc. Miami


Could you folks please let me know what methods and reagents you have 
worked with that do a decent job of overnight clearing the fat from 
colonectomy specimens to further reveal lymph nodes? 

Many Thanks,

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