[Histonet] RE: Revealing lymph nodes
Hartman's fixative (formerly Davidson's fixative)works about the best for me. It is commercially available from MarketLab and BBC Biochemical to name a few. To prepare it yourself, mix the following -
95% ETOH 1200 ml
37% Formaldehyde 800 ml
DH20 1200 ml
Glacial Acetic Acid 400 ml
The tissue can remain in the fixative for up to 24 hours. Transfer to 10% NBF. The nodes begin to turn opaque-white and reveal themselves after about 30 minutes in this fixative.
Eric C. Kellar
Quest Diagnostics Inc. Miami
Could you folks please let me know what methods and reagents you have
worked with that do a decent job of overnight clearing the fat from
colonectomy specimens to further reveal lymph nodes?
The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited.
Histonet mailing list
<< Previous Message | Next Message >>