[Histonet] RDO question


Good morning Histonet!

We are using RDO (a rapid decalcifier with hydrochloric acid) in our
laboratory to decalcify very high density ear bones that surround the
inner ear of marine mammals. The inner ear cells are fixed with formalin
10% and this is the soft tissue we are interested in.

Our problem is that the bone surrounding the inner ear is not homogeneous.
So, while some points are decalcified after 16 hours other
need much more time. Then the inner ear cells are in contact with the RDO
much before the sample is completely decalcified.

I would like to ask if someone know how is the reaction we can expect from
the cells fixed in formalin in contact with RDO.
I'm wondering what you can advise me to do in our case, when the bone is
not homogeneous, but it has to be decalicified.

Thank you very much for your help.

Best regards from,
Maria Morell

Maria Morell
Laboratori d'Aplicacions Bioacústiques
Centre Tecnològic de Vilanova i la Geltrú
Universitat Politècnica de Catalunya
Avda. Rambla Exposició s/n
08800-Vilanova i la Geltrú (Barcelona)- Spain
Telf: 0034 938967227

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