[Histonet] ICAM-1, CD54

From:"Donna Harclerode"

Hi Rachael


I am not familiar with the Serotech antibody, but PharMingen has an anti
mouse CD54 (ICAM-1) made in Armenian hamster link
led/74121E_550287.pdf  that works great in frozen mouse sections at
5ug/ml.  You do need to purchase the Armenian hamster secondary for this
antibody cat 550335 (biotinolated anti Armenian hamster) and use a LSAB,
ABC or other tertiary with DAB. 


PharMingen makes a lot of antibodies directed against mice (humans and
rats too), mostly CD markers, that work very well in IHC.  If you are
looking for any other specific targets, let me know and I can check my
data sheets for you (I generated data in 97 for all the PharMingen
antibodies that were available then for IHC applications).  I could also
send the sheets to you if you wish.  The catalog numbers have all
changed since 97, but the clones are still the same. 


Good luck,


Donna Harclerode, HT, (ASCP), HTL, QIHC

Scientist / Immunohistochemistry

Cytori Therapeutics

3020 Callan Rd.

San Diego, CA 92121

858-458-0900 ext 322



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