[Histonet] Ehrlich Biondi Heidenhain stain


Hi all,

does someone know the protocol for staining with the “Ehrlich Biondi Heidenhain” method?
Particularly how long has the specimen to stay in the staining solution?
I have noticed, in previous, tests that is quit difficult to dye the nucleus with the methyl green contained in the solution.
I am processing thin sections of mouse liver included in wax and fixed with Bouin liquid.
I also wonder why the sections are crunched after cutting. There is a solution at this problem?

Thanks in advance.
Best Regards,


Università degli Studi di Pisa
Facoltà di Ingegneria Chimica

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