[Histonet] Blue spots
Help! I am seeing blue spots !!!? I have completed a BDA/DAB reaction
on some rabbit brain tissue. When I used Cresyl Violet for a
counterstain - I had small blue "ponds" randomly showing up on the
tissue. I have used the same method for years and had not gotten this
result? Basic info: tissue is paraformaldehyde fixed and cut as frozen
sections @ 50 microns. Used same batch of CV that has been used in the
past. The rest of the tissue looks perfectly fine. Any ideas as to
what went wrong?
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