Re: [Histonet] water for frozen section staining

From:Rene J Buesa

We always used running water at room temp. (sometimes quite "cold"). If you use warm/hot water maybe the section will "blue" sooner but just because the added effect of above room temp. of the water on the speed of the reaction.
  If temperature is going to affect the section adherence to the slide, it is probably the warm/hot water the one that could affect detrimentally the most.
  René J.

"Kapoor, Sue"  wrote:
  Hello histonetters,

We have a debate going in my lab as to using warm/hot or cold running water
when staining frozen sections. One side states warm/hot water "blues" the
hematoxylin...the other sides feels the cold water keeps the sections from
falling off.

What does everyone in histo land do and think???

thanks for your replies :)

Sue Kapoor, HT (ASCP)
Histology Coordinator
Kenosha Medical Center
Kenosha, WI

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